As he signed a bill giving the state control over Disney's expansive Orlando theme parks, Florida Governor Ron DeSantis urged people to "buckle up.".
The legislation gives Mr. DeSantis' Republican-led legislature more oversight while taking away Disney's 50-year-old exclusive power.
Disney opposed state laws that restricted gender and sexuality education, so the action is perceived as retaliation.
It comes before Mr. DeSantis' likely presidential run in 2024.
At a bill signing event on Monday in Lake Buena Vista, Florida, close to Walt Disney World, he declared: "Today, the corporate kingdom finally comes to an end.".
"There is a new sheriff in town, and responsibility will rule the day. ".
The expansive Walt Disney World property operated within Florida's Reedy Creek Improvement District for more than 50 years and essentially served as a self-governing area with control over utilities and a fire department.
This, it had been argued, spared the local tax payers the expense of maintaining the 43 square miles (111 sq km) of property that draws millions of visitors each year.
The new law does not dissolve the district or shift its sizable debt to taxpayers, but it does subject Disney to more layers of outside scrutiny through a five-member board that is now appointed by the state.
Additionally, it means that Disney will be subject to the same state regulations as other theme parks in Orlando, including building and fire safety codes, according to Mr. DeSantis.
Martin Garcia, a Tampa attorney and Republican donor whose firm gave $50,000 (£41,467) to Mr. DeSantis' reelection campaign, was named to the new board when Mr. DeSantis announced the appointments.
In addition, Christian Ziegler, the new chairman of the Republican Party of Florida, appointed Bridget Ziegler, a member of the Sarasota County School Board and co-founder of the conservative group Moms for Liberty.
Next week is the first meeting of the new board, according to Mr. DeSantis, who also warned that "you better buckle up" because they will be in charge.
In the newly created Central Florida Tourism Oversight District, one of the first actions Mr. DeSantis suggested would be taken was to increase the pay of emergency first responders.
Disney, one of the largest media companies in the world, has been at odds with Florida lawmakers since Mr. DeSantis's controversial bill was signed into law last April.
Disney expressed its opposition to the legislation in a public statement, which prohibited gender and sexuality education for students nine years of age and younger in Florida schools.
The law, which some have dubbed the "Don't Say Gay" law and which has since been passed, has drawn criticism for isolating and stigmatizing LGBT youth, while its proponents argue that it will shield kids from inappropriate online content.
DeSantis urged lawmakers to deprive Disney of its unique governing authority in response to the opposition.
The controversy between Mr. DeSantis and Disney in the public eye has raised his profile as a prospective Republican presidential candidate in 2024.
DeSantis did, however, make a suggestion that he was still a fan of the Mouse during a news conference on Monday. I've always been very proud of our parks, he said, "in spite of everything that's happened over the last couple of years.".
Many families have had wonderful experiences coming down here, and it's almost like a rite of passage for them. But you need allies who will be honest with you when you're lost.
. "